Chromacon

Capture SMB

Summary

Introduction

Affinity capture chromatography is a crucial step for the efficient processing of large volumes of cell culture harvest. However, despite suboptimal process performance, single-column batch chromatography is still standard due to it's simplicity. The superior alternative to single-column batch processing is Periodic CounterCurrent (PCC) purification, which offers significant economic benefits. ChromaCon has developed an optimized twin-column PCC process called CaptureSMB, the most effective and least complex PCC process available to date. To facilitate CaptureSMB development, we offer the Contichrom CUBE, a specialized twin-column FPLC system with a CaptureSMB wizard for software aided method creation.

How does CaptureSMB work?

Unlike batch chromatography, which uses only 50-60% of resin material, CaptureSMB fully loads one column and captures product breakthrough on a second interconnected column, ensuring maximum product capture and efficient utilization of all available affinity resin (see process principle). This technology offers two-fold faster processing times and up to 50% savings in Protein A resin and buffer.

How does CaptureSMB compare to 3 or more column processes?

With only two columns, CaptureSMB has the least complex system configuration compared to multi-column systems, providing a de-risked operation while still benefiting from continuous processes. CaptureSMB has been implemented at an industrial scale on Contichrom TWIN GMP systems and has demonstrated superior performance in recent publications . Furthermore, a virus clearance study has shown that the CaptureSMB process has equivalent virus clearance compared to a batch process.

CaptureSMB Resources

 

Application Notes (pdf)

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Links to Selected Publications

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Case Studies (ppt)

Comparison of CaptureSMB, 3C-PCC and 4C-PCC productivity

Continuous capture processes are superior to bach processes. CaptureSMB provides the advantage in combination with the lowest complexityContinuous capture processes are superior to bach processes. CaptureSMB provides the advantage in combination with the lowest complexity

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